Tetrahymena pyriformis Phagocytic Activity Test for Rapid Toxicity Assessment of Aquatic Micropollutants

  • Ildikó Fekete-Kertész Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary
  • Orsolya Ullmann Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary
  • Panni Csizmár Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary
  • Mónika Molnár Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary

Abstract

The increasing worldwide contamination of freshwater ecosystems with micropollutants such as pharmaceuticals, personal care products, hormones, industrial chemicals and pesticides is a key environmental problem facing humanity nowadays. In order to assess the adverse effect of six organic micropollutants (atrazine, bisphenol A, dibutyl phthalate, 17β-estradiol, 3,4-dichlorophenol and Na-diclofenac) a short term phagocytic activity test was applied with Tetrahymena pyriformis representing an important trophic level of aquatic ecosystems.
Significant concentration-dependent response in phagocytic activity was experienced to 17β-estradiol and other endocrine disrupting compounds such as 3,4-dichlorophenol, atrazine and dibutyl phthalate with lowest observed effect concentration of 0.01; 0.005; 0.05; 0.05 µg/L, respectively. Although bisphenol A and Na-diclofenac did not result in a concentration-dependent response, significant changes in phagocytic activity were observed.
Our results suggest that the phagocytic activity assay relying on a basic cell response can be a valuable method for the early and sensitive indication of adverse effects of micropollutants.

Keywords

ecotoxicity, micropollutants, phagocytic activity, Tetrahymena pyriformis, xenoestrogens
Published
31-08-2017
How to Cite
FEKETE-KERTÉSZ, Ildikó et al. Tetrahymena pyriformis Phagocytic Activity Test for Rapid Toxicity Assessment of Aquatic Micropollutants. Periodica Polytechnica Chemical Engineering, [S.l.], aug. 2017. ISSN 1587-3765. Available at: <https://pp.bme.hu/ch/article/view/10667>. Date accessed: 24 sep. 2017. doi: https://doi.org/10.3311/PPch.10667.
Section
Articles