Hydrothermal Synthesis of Sr-doped Hydroxyapatite and Its Antibacterial Activity

  • Teodóra Nagyné-Kovács Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
  • Boglárka Mészáros Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
  • Mónika Molnár Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
  • Mária Tolner Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
  • István Endre Lukács Institute for Technical Physics and Materials Science, Centre for Energy Research, Hungarian Academy of Sciences, H-1121 Budapest, Konkoly Thege M. út 29-33, Hungary
  • Imre M. Szilágyi Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
  • György Pokol Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary; Research Centre for Natural Sciences, Hungarian Academy of Sciences, H-1117 Budapest, Magyar tudósok körútja 2., Hungary

Abstract

In this study, we prepared hydroxyapatite (HAP) samples using hydrothermal method. We investigated the effect of reaction conditions such as phosphate excess applying 1.49 and 1.67 (as stoichiometric) Ca/P ratio, pH (9/ 10/ 11/ 12) and time (4/ 8/ 12/ 24 h). Sample characterization was carried out by XRD and SEM. The results showed, all samples had HAP structure, however, lower Ca/P ratio, larger reaction time and setting the pH to 10 increased the crystallinity. Then, we synthetized Sr-doped HAP samples, varying the Sr concentration using 2, 4, 6, 8 and 10 % Sr/ (Ca+Sr). The Sr content was revealed by EDX. Sr-incorporation did not change the obtained crystalline HAP phase but the unit cell parameters increased. We calculated lattice constants and found that a, b changed from 9.4310 Å to 9.4700 Å, c from 6.8819 Å to 6.9227 Å and the unit cell volume from 530.0951 Å3 to 537.6556 Å3 due to the larger ionic radius of Sr compared to Ca. The pure and doped samples had uniform, mostly needle-like morphology with 100-300 nm length and 25-100 nm. In vitro cytotoxicity tests revealed evident antibacterial activity in the case of doped samples compared to pure HAP against E. coli.

Keywords: Sr-doped hydroxyapatite, hydrothermal, nanoparticles, lattice parameters, antibacterial activity
Published online
2019-09-11
How to Cite
Nagyné-Kovács, T., Mészáros, B., Molnár, M., Tolner, M., Lukács, I., Szilágyi, I. and Pokol, G. (2020) “Hydrothermal Synthesis of Sr-doped Hydroxyapatite and Its Antibacterial Activity”, Periodica Polytechnica Chemical Engineering, 64(1), pp. 54-60. doi: https://doi.org/10.3311/PPch.14062.
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Articles