Conservation of the Biocatalytic Activity of Whole Yeast Cells by Supported Sol – Gel Entrapment for Efficient Acyloin Condensation

  • László Nagy-Győr Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary
  • Emese Farkas Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary
  • Mihai Lăcătuș Biocatalysis and Biotransformation Research Center, Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania
  • Gergő Tóth Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary
  • Dániel Incze Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary
  • Gábor Hornyánszky Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary
  • Viktória Bódai Fermentia Ltd, H-1045 Budapest, Berlini str. 45-47., Hungary; Witaria Ltd, H-1087 Budapest, Luther str. 4-6, Hungary
  • Csaba Paizs Biocatalysis and Biotransformation Research Center, Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania
  • László Poppe Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary; Biocatalysis and Biotransformation Research Center, Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Ro-400028 Cluj-Napoca, Romania; SynBiocat LLC, H-1172 Budapest, Szilasliget str. 3., Hungary
  • Diána Balogh-Weiser Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary; Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P. O. B. 91, Hungary

Abstract

In this study, an efficient and generally applicable 2nd generation sol – gel entrapment method was developed for immobilization of yeast
cells. Cells of Lodderomyces elongisporus, Candida norvegica, Debaryomyces fabryi, Pichia carsonii strains in admixture with hollow silica microspheres support were immobilized in sol – gel matrix obtained from polycondensation of tetraethoxysilane. As biocatalysts in the
selective acyloin condensation of benzaldehyde catalyzed by pyruvate decarboxylase of the yeast, the novel immobilized whole-cell preparations were compared to other states of the cells such as freshly harvested wet cell paste, lyophilized cells and sol – gel entrapped preparations without hollow silica microspheres support. Reusability and storability studies designated this novel 2nd generation sol –
gel method as a promising alternative for solid formulation of whole-cells bypassing expensive and difficult downstream steps while providing easy-to-handle and stable biocatalysts with long-term preservation of the biocatalytic activity.

Keywords: yeast, whole-cell, immobilization, biocatalysis, acyloin condensation
Published online
2019-12-12
How to Cite
[1]
Nagy-Győr, L., Farkas, E., Lăcătuș, M., Tóth, G., Incze, D., Hornyánszky, G., Bódai, V., Paizs, C., Poppe, L. and Balogh-Weiser, D. (2020). Conservation of the Biocatalytic Activity of Whole Yeast Cells by Supported Sol – Gel Entrapment for Efficient Acyloin Condensation. Periodica Polytechnica Chemical Engineering. 64, 2 (2020), 153-161. https://doi.org/10.3311/PPch.14645.
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Articles