The Role of the Initiator System in the Synthesis of Acidic Multifunctional Nanoparticles Designed for Molecular Imprinting of Proteins

Authors

  • Marwa Aly Ahmed
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Szent Gellért tér 4., Hungary
    Department of Chemistry, Faculty of Science, Arish University, 45511 El-Arish, North Sinai, Dahyet El Salam, Egypt
  • Júlia Erdőssy
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Szent Gellért tér 4., Hungary
  • Viola Horváth
    Affiliation
    Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Szent Gellért tér 4., Hungary
    MTA-BME Computation Driven Chemistry Research Group, H-1111 Budapest, Szent Gellért tér 4., Hungary
https://doi.org/10.3311/PPch.15414

Abstract

Multifunctional nanoparticles have been shown earlier to bind certain proteins with high affinity and the binding affinity could be enhanced by molecular imprinting of the target protein. In this work different initiator systems were used and compared during the synthesis of poly (N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) nanoparticles with respect to their future applicability in molecular imprinting of lysozyme. The decomposition of ammonium persulfate initiator was initiated either thermally at 60 °C or by using redox activators, namely tetramethylethylenediamine or sodium bisulfite at low temperatures. Morphology differences in the resulting nanoparticles have been revealed using scanning electron microscopy and dynamic light scattering. During polymerization the conversion of each monomer was followed in time. Striking differences were demonstrated in the incorporation rate of acrylic acid between the tetramethylethylenediamine catalyzed initiation and the other systems. This led to a completely different nanoparticle microstructure the consequence of which was the distinctly lower lysozyme binding affinity. On the contrary, the use of sodium bisulfite activation resulted in similar nanoparticle structural homogeneity and protein binding affinity as the thermal initiation.

Keywords:

poly(N-isopropylacrylamide) nanoparticle, tetramethylethylenediamine, sodium bisulfite, initiator, protein binding

Citation data from Crossref and Scopus

Published Online

2020-08-24

How to Cite

Ahmed, M. A., Erdőssy, J., Horváth, V. “The Role of the Initiator System in the Synthesis of Acidic Multifunctional Nanoparticles Designed for Molecular Imprinting of Proteins”, Periodica Polytechnica Chemical Engineering, 65(1), pp. 28–41, 2021. https://doi.org/10.3311/PPch.15414

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