Cornea-PAMPA as an Orthogonal in Vitro Physicochemical Model of Corneal Permeability

  • Anna Vincze
    Affiliation

    Department of Chemical and Environmental Process Engineering, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary

  • Gergő Dargó
    Affiliation

    Department of Chemical and Environmental Process Engineering, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary

  • György Tibor Balogh
    Affiliation

    Department of Chemical and Environmental Process Engineering, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rakpart 3., Hungary
    Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, H-6720 Szeged, Zrínyi u. 9., Hungary

Abstract

The present study was aimed to investigate the relationships between permeability and membrane retention values (logPe and MR) of the in vitro non-cellular permeability assay, corneal-PAMPA in comparison with experimental Caco-2 permeability data and calculated physicochemical properties (MW, clogP, clogD7.4 , TPSA). For the investigation, 50 structurally and physicochemically diverse drugs were selected and measured in PAMPA model optimized for corneal permeability. The results showed corneal-PAMPA model's orthogonality in terms of passive diffusion to the FDA approved Caco-2 as a gastrointestinal absorption model, while the comparison with physicochemical properties revealed trends between logPe , MR and the lipophilicity descriptors and TPSA.

Keywords: corneal-PAMPA, permeability, membrane retention, physicochemical descriptors, clogP/D7.4, TPSA
Published online
2020-05-25
How to Cite
Vincze, A., Dargó, G., Balogh, G. T. “Cornea-PAMPA as an Orthogonal in Vitro Physicochemical Model of Corneal Permeability”, Periodica Polytechnica Chemical Engineering, 64(3), pp. 384-390, 2020. https://doi.org/10.3311/PPch.15601
Section
Articles