Increase in Fermentable Sugars of Olive Tree Pruning Biomass for Bioethanol Production: Application of an Experimental Design for Optimization of Alkaline Pretreatment

Authors

  • Arminda Mamaní
    Affiliation

    Institute of Chemical Engineering, Faculty of Engineering, National University of San Juan, Av. Libertador 1109, J5400 San Juan, Argentina
    National Council of Scientific and Technical Research (CONICET), Godoy Cruz 2290, C1425FQB Buenos Aires, Argentina

  • Yolanda Maturano
    Affiliation

    Biotechnology Institute, Faculty of Engineering, National University of San Juan, Av. Libertador 1109, J5400 San Juan, Argentina
    National Council of Scientific and Technical Research (CONICET), Godoy Cruz 2290, C1425FQB Buenos Aires, Argentina

  • Laura Herrero
    Affiliation

    Institute of Chemical Engineering, Faculty of Engineering, National University of San Juan, Av. Libertador 1109, J5400 San Juan, Argentina
    National Council of Scientific and Technical Research (CONICET), Godoy Cruz 2290, C1425FQB Buenos Aires, Argentina

  • Laura Montoro
    Affiliation

    Institute of Chemical Engineering, Faculty of Engineering, National University of San Juan, Av. Libertador 1109, J5400 San Juan, Argentina
    National Council of Scientific and Technical Research (CONICET), Godoy Cruz 2290, C1425FQB Buenos Aires, Argentina

  • Fabiana Sardella
    Affiliation

    Institute of Chemical Engineering, Faculty of Engineering, National University of San Juan, Av. Libertador 1109, J5400 San Juan, Argentina

https://doi.org/10.3311/PPch.18247

Abstract

Olive Tree Pruning (OTP) biomass can be considered a suitable source of fermentable sugars for the production of second-generation bioethanol. The present study proposes a remarkable alternative for the valorization of olive tree pruning residues. OTP biomass was processed using a sequential calcium hydroxide pretreatment/enzymatic hydrolysis. A 24–1 half fractional factorial design was adopted for the screening of process variables and a central composite design was used for the optimization stage. Temperature and lime loading resulted statistically significant. The following optimal conditions were obtained: 0.01 g of Ca(OH)2/g of dry material, 20 g of H2O/g of dry material at 160 °C for 2 h. The mathematical model that governs this alkaline pretreatment was obtained with a 76% adjusted determination coefficient, which means that it is a good representation of the process. Under optimal operating conditions, 13% of the cellulose and 88% of the hemicellulose was solubilized. Moreover, the fermentable sugar content increased 1800% compared with the initial conditions, obtaining 240 g of glucose per kg of OTP residue. The fermentable sugars obtained after the calcium hydroxide pretreatment and enzymatic hydrolysis of OTP biomass yielded 2.8 g of ethanol/100 g of raw material.

Keywords:

Olive Tree Pruning (OTP), calcium hydroxide pretreatment, optimum conditions, fermentable sugars, bioethanol

Published Online

2022-02-15

How to Cite

Mamaní, A., Maturano, Y., Herrero, L., Montoro, L., Sardella, F. “Increase in Fermentable Sugars of Olive Tree Pruning Biomass for Bioethanol Production: Application of an Experimental Design for Optimization of Alkaline Pretreatment”, Periodica Polytechnica Chemical Engineering, 66(2), pp. 269–278, 2022. https://doi.org/10.3311/PPch.18247

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Section

Articles