Immobilization of lipases from Rhizomucor miehei and Thermomyces lanuginosus by adsorption on variously grafted silica gels

Abstract

Twenty variously surface-modified mesoporous silica gels were studied as carriers for immobilization by hydrophobic adsorption of the lipases from Rhizomucor miehei (RmL) and Thermomyces lanuginosus (TlL). Several of the surfacemodified silica gels studied proved to be advantageous supports for RmL and TlL resulting in novel biocatalysts of high activity and enantioselectivity in the kinetic resolution of racemic 1-phenylethanol rac-1. The fact that it were different grafting methods which led to the most efficient supports for RmL and TlL indicated that the selection of optimal support for the immobilization of a particular lipase cannot be predicted.