VARIETY IDENTIFICATION OF SOME RICE CULTIVARS BY POLYACRYLAMIDE GEL ELECTROPHORESIS

Abstract

Proteins of 5 rice cultivars grown in Hungary and 7 varieties grown in Vietnam were studied. After defatting of the samples with hexane or hexane and chloroform-methanol (1:1) mixture, the proteins were extracted with a TRIS-glycine buffer (pH=6.8) containing SDS and beta-mercaptoethanol. The protein extract was investigated by SDS-PAGE. Significant differences were found both in subunit distribution and intensities of the patterns making possible the identification of varieties.