Cross-linking of Horseradish Peroxidase Enzyme to Fine Particles Generated by Nano Spray Dryer B-90

Abstract

Enzymatic methods can be effective tools for removal of phenolic compounds from wastewater by forming reactive radicals with polymerization mechanism. Comparing with conventional chemical ways, enzymes have advantages such as low cost and high level of catalytic efficiency. At the same time, there exist some problems, e.g. the instability of enzyme structures, sensitivity to process conditions and their purification cost is high. Fine particles have been used for enzyme stabilization due to their beneficial high surface-to-volume ratio. Enzymes immobilized on fine particles may work at a broader pH, temperature and salinity range than the native enzymes. Ethyl cellulose and poly(lactic co-glycolic acid) carrier particles were prepared by nano spray drying and used for immobilizing horseradish peroxidase enzyme (HRP) by cross-linking. The optimal condition for immobilization was investigated. Fixed enzyme showed increased activity in TRIS buffer. The activity of the immobilized enzyme was enhanced at acidic and alkaline pH.